4BG9
Apo form of a putative sugar kinase MK0840 from Methanopyrus kandleri (orthorhombic space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.043, 115.967, 48.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.805 - 1.902 |
| R-factor | 0.1802 |
| Rwork | 0.179 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BG8 CHAIN A |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.375 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.800 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.710 |
| Number of reflections | 33085 | |
| <I/σ(I)> | 14.6 | 2.17 |
| Completeness [%] | 99.5 | 97.5 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 0.5 M POTASSIUM THIOCYANATE, 0.1 M SODIUM ACETATE PH 4.6 AT 293 K |
