4BFO
Crystal Structure of the Starch-Binding Domain from Rhizopus oryzae Glucoamylase in Complex with isomaltotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.529, 25.866, 61.085 |
| Unit cell angles | 90.00, 124.01, 90.00 |
Refinement procedure
| Resolution | 25.318 - 1.175 |
| R-factor | 0.1571 |
| Rwork | 0.156 |
| R-free | 0.16930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8l |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.189 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.210 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.040 | 0.170 |
| Number of reflections | 36109 | |
| <I/σ(I)> | 30.25 | 6.44 |
| Completeness [%] | 97.8 | 86.2 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M NA-CITRATE PH 5.6, 18% PEG4000, 0.2 M NH4-ACETATE |
