4BFG
Structure of the extracellular portion of mouse CD200R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 51.970, 51.970, 175.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.080 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bfe |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.840 | 2.130 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.080 | 0.740 |
| Number of reflections | 15423 | |
| <I/σ(I)> | 16.8 | 3.1 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 7.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 15% PEG4000, 0.1M SODIUM ACETATE, 0.2M AMMONIUM ACETATE |
