4BFE
Structure of the extracellular portion of mouse CD200RLa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 157.590, 157.590, 167.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.1731 |
| Rwork | 0.171 |
| R-free | 0.20530 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 168.020 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.490 |
| Number of reflections | 42242 | |
| <I/σ(I)> | 13 | 2.2 |
| Completeness [%] | 98.4 | 99.7 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.0M AMMONIUM SULFATE, 0.1M SODIUM CACODYLATE, 0.2M SODIUM CHLORIDE, pH 6.5 |
