4BEZ

Night blindness causing G90D rhodopsin in the active conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	H 3 2
Unit cell lengths	242.440, 242.440, 110.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.300
R-factor	0.2299
Rwork	0.229
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a4m
RMSD bond length	0.011
RMSD bond angle	1.601
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (MR)
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.470
High resolution limit [Å]	3.300	3.300
Rmerge	0.310
Number of reflections	18833
<I/σ(I)>	9	1.43
Completeness [%]	99.4	97.4
Redundancy	20.2	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1