4BEX
Structure of human Cofilin1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.940, 85.940, 85.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.130 - 2.800 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cof |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.670 |
| Number of reflections | 9292 | |
| <I/σ(I)> | 5.7 | 1.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.4 M SODIUM MALONATE PH 7.0 THE CRYSTALS WERE AIR-DRIED BEFORE FREEZING TO DEHYDRATE. |
