4BDM
Crystal structure of the GluK2 K531A LBD dimer in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.688, 101.019, 126.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.970 - 3.400 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.28865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xxr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.479 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.970 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.270 | 0.640 |
| Number of reflections | 14147 | |
| <I/σ(I)> | 6.6 | 2.5 |
| Completeness [%] | 94.7 | 95.1 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 21% PEG 4,000, 9% PROPAN-2-OL, 80MM NA ACETATE |
