4BDL
Crystal structure of the GluK2 K531A LBD dimer in complex with glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 97.568, 106.611, 113.904 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.924 - 1.750 |
| R-factor | 0.1709 |
| Rwork | 0.169 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xxr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.028 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.920 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.770 |
| Number of reflections | 59953 | |
| <I/σ(I)> | 15.5 | 2.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 19% PEG 4,000, 3% PROPAN-2-OL, 80MM NA ACETATE |
