4BCZ
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-15 |
| Detector | MARRESEARCH 165MM |
| Spacegroup name | P 65 |
| Unit cell lengths | 74.430, 74.430, 59.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.930 |
| R-factor | 0.1718 |
| Rwork | 0.169 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.121 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.060 | 0.650 |
| Number of reflections | 14115 | |
| <I/σ(I)> | 21.4 | 3.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 30% PEG 1500, 0.1M MIB BUFFER, PH 4.0, 20 C |
