4BCU
Satellite Tobacco Necrosis Virus (STNV) virus like particle in complex with the B3 aptamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 315.020, 300.550, 183.510 |
| Unit cell angles | 90.00, 94.37, 90.00 |
Refinement procedure
| Resolution | 217.150 - 2.290 |
| R-factor | 0.15729 |
| Rwork | 0.155 |
| R-free | 0.19821 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.756 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 217.000 | 2.350 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 756410 | |
| <I/σ(I)> | 9.3 | 3.3 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 5.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
