4BCT
Crystal structure of kiwi-fruit allergen Act d 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.541, 48.312, 50.580 |
| Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
| Resolution | 50.000 - 0.980 |
| R-factor | 0.11406 |
| Rwork | 0.113 |
| R-free | 0.13125 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.394 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 0.980 |
| Rmerge | 0.040 |
| Number of reflections | 97776 |
| <I/σ(I)> | 29.7 |
| Completeness [%] | 99.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22-30% PEG 6000, PH 6.8-7.5 |
