4BA6
High Resolution structure of the C-terminal family 65 Carbohydrate Binding Module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 83.570, 83.570, 36.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.370 - 1.420 |
| R-factor | 0.17121 |
| Rwork | 0.170 |
| R-free | 0.19728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4afm |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.495 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.750 | 1.500 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.060 | 0.690 |
| Number of reflections | 27755 | |
| <I/σ(I)> | 11.9 | 1.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 292 | 2M AMMONIUM SULPHATE, 292 K, pH 7 |
