4B9T
Structure of the high fidelity DNA polymerase I with an oxidative formamidopyrimidine-dG DNA lesion -dC basepair in the post-insertion site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.381, 93.775, 105.608 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.650 |
| R-factor | 0.22395 |
| Rwork | 0.221 |
| R-free | 0.27302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u45 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 24741 | |
| <I/σ(I)> | 17.6 | 4 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 5.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
