4B97
Biomass sensing modules from putative Rsgi-like proteins of Clostridium thermocellum resemble family 3 carbohydrate-binding module of cellulosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.479, 56.043, 64.594 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.331 - 1.276 |
| R-factor | 0.1405 |
| Rwork | 0.140 |
| R-free | 0.15640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wnx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.502 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 33242 | |
| <I/σ(I)> | 30.8 | 2.3 |
| Completeness [%] | 99.8 | 96.7 |
| Redundancy | 6.83 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.2 M AMMONIUM ACETATE, 0.1 M TRI-SODIUM CITRATE PH 5.6, 30 % (V/V) PEG 400 |
