4B92
Crystal structure of truncated human CRMP-5 soaked with Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.500, 89.500, 246.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.920 - 2.900 |
| R-factor | 0.19377 |
| Rwork | 0.191 |
| R-free | 0.25009 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b91 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.459 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.140 | 0.650 |
| Number of reflections | 23108 | |
| <I/σ(I)> | 10.1 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 23% (V/V) PEG 400 MMT PH 5.0 |
