4B8S
Crystal Structure of Thermococcus litoralis ADP-dependent Glucokinase (GK)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-10 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 106.468, 106.468, 130.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.850 - 2.580 |
R-factor | 0.17198 |
Rwork | 0.169 |
R-free | 0.22056 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gc5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.350 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.650 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.090 | 0.670 |
Number of reflections | 33911 | |
<I/σ(I)> | 18.3 | 3.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.2 | 1.5 M SODIUM CITRATE PH 5.2, 5 MM DTT, 6 MM MG-ADPBETAS AND 30 MM D-GLC. |