4B8R
Crystal Structure of Thermococcus litoralis ADP-dependent Glucokinase (GK)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 109.104, 109.104, 129.559 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.280 - 2.050 |
| R-factor | 0.17168 |
| Rwork | 0.170 |
| R-free | 0.21093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gc5 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.058 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.090 | 0.560 |
| Number of reflections | 72183 | |
| <I/σ(I)> | 19.4 | 3.7 |
| Completeness [%] | 94.7 | 99.2 |
| Redundancy | 9.4 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.6 | 14 % (W/V) POLYETHYLENE GLYCOL (PEG) 6000, 0.2 M LISO4, 0.1 M SODIUM CITRATE PH 3.6 AND 5 MM DITHIOTHREITOL (DTT). |
