4B8P
rImp_alpha_A89NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.425, 140.967, 73.167 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 19.818 - 2.300 |
| R-factor | 0.1896 |
| Rwork | 0.189 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ee4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.820 | 19.820 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.150 | 0.480 |
| Number of reflections | 55804 | |
| <I/σ(I)> | 4.5 | 2 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M BIS-TRIS PROPANE PH 7.0, 15% PEG 3350, 0.2 M NDSB-221 AND 0.2 M NAF |
