4B8D

TENSEGRITY TRIANGLE FROM ENZYMATICALLY MANUFACTURED DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-25
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	H 3
Unit cell lengths	106.437, 106.437, 95.154
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.280 - 4.790
R-factor	0.1872
Rwork	0.185
R-free	0.20460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.010
RMSD bond angle	2.460
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.300	5.050
High resolution limit [Å]	4.790	4.790
Rmerge	0.050	0.800
Number of reflections	1980
<I/σ(I)>	16.3	2.3
Completeness [%]	99.9	100
Redundancy	5.8	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1