4B7T

Glycogen Synthase Kinase 3 Beta complexed with Axin Peptide and Leucettine L4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	81.577, 81.577, 283.654
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.986 - 2.772
R-factor	0.1973
Rwork	0.195
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o9u
RMSD bond length	0.002
RMSD bond angle	0.585
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.990	2.940
High resolution limit [Å]	2.770	2.770
Rmerge	0.060	0.270
Number of reflections	14709
<I/σ(I)>	23.1	7.1
Completeness [%]	97.3	91.1
Redundancy	7.2	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M TRIS-HCL, PH 8.0, 0.15 M MGCL2, 15% (W/V) PEG 4000