4B7T
Glycogen Synthase Kinase 3 Beta complexed with Axin Peptide and Leucettine L4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.577, 81.577, 283.654 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.986 - 2.772 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9u |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.585 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.990 | 2.940 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.060 | 0.270 |
| Number of reflections | 14709 | |
| <I/σ(I)> | 23.1 | 7.1 |
| Completeness [%] | 97.3 | 91.1 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL, PH 8.0, 0.15 M MGCL2, 15% (W/V) PEG 4000 |
