4B67
A. fumigatus ornithine hydroxylase (SidA), re-oxidised state bound to NADP and ornithine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.170, 84.040, 144.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.670 - 2.750 |
| R-factor | 0.21731 |
| Rwork | 0.214 |
| R-free | 0.28521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b63 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.985 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.150 | 0.460 |
| Number of reflections | 12219 | |
| <I/σ(I)> | 5.2 | 2 |
| Completeness [%] | 97.6 | 97.3 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | HANGING DROP VAPOR DIFFUSION METHOD, WITH A RESERVOIR SOLUTION CONTAINING; 1.6 M AMMONIUM SULFATE, 0.1 M HEPES, 2% DIOXANE, PH 6.6. |
