4B65
A. fumigatus ornithine hydroxylase (SidA), reduced state bound to NADP(H)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14 |
| Synchrotron site | ESRF |
| Beamline | ID14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.990, 84.460, 145.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.810 - 2.320 |
| R-factor | 0.19877 |
| Rwork | 0.196 |
| R-free | 0.25013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b63 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.109 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.080 | 0.210 |
| Number of reflections | 21091 | |
| <I/σ(I)> | 11.8 | 5 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 4.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | HANGING DROP VAPOR DIFFUSION METHOD, WITH A RESERVOIR SOLUTION CONTAINING; 1.6 M AMMONIUM SULFATE, 0.1 M HEPES, 2% DIOXANE, PH 6.6. |
