4B5O
Crystal structure of human alpha tubulin acetyltransferase catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-10 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 42.198, 121.958, 37.277 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.649 - 1.050 |
R-factor | 0.132 |
Rwork | 0.131 |
R-free | 0.15740 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.021 |
RMSD bond angle | 2.086 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.060 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.030 | 0.790 |
Number of reflections | 86586 | |
<I/σ(I)> | 29 | 2.3 |
Completeness [%] | 1.0 | 1 |
Redundancy | 7.6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 50 MM TRIS PH 8.0, 200 MM NAC, 15% PEG8000 |