4B5H
Substate bound inactive mutant of Neisseria AP endonuclease in presence of metal ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.907, 68.907, 120.968 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.065 - 3.050 |
R-factor | 0.1962 |
Rwork | 0.185 |
R-free | 0.29490 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.003 |
RMSD bond angle | 0.528 |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 59.800 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.100 |
Number of reflections | 5920 |
Completeness [%] | 99.2 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |