4B56
Structure of ectonucleotide pyrophosphatase-phosphodiesterase-1 (NPP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-03 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.385, 105.412, 245.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.470 - 3.000 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xrg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.470 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | 0.640 |
| Number of reflections | 51597 | |
| <I/σ(I)> | 17.1 | 2.1 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 NL PROTEIN SOLUTION WITH 100 NL 10% PROPANOL, 0.1 M IMIDAZOLE AT PH 8.0 AND 3% 1,4-DIOXANE |
