4B46

CetZ1 from Haloferax volcanii - GDP bound monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-02
Detector	ADSC QUANTUM CCD
Spacegroup name	P 21 21 21
Unit cell lengths	56.339, 62.214, 96.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.654 - 1.900
R-factor	0.1753
Rwork	0.174
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARTIAL MODEL OF CETZ4
RMSD bond length	0.006
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.650	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.110	0.580
Number of reflections	27386
<I/σ(I)>	10	1.9
Completeness [%]	99.8	99.4
Redundancy	4.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4		20% W/V PEG 6K, 1 M LITHIUM CHLORIDE, 0.1 M NA CITRATE PH 4