4B43
Bacterial translation initiation factor IF2 (1-363), apo form, double mutant K86L H130A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 295 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.787, 61.971, 162.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.283 - 1.937 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b3x |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.367 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1278)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.080 | 0.890 |
| Number of reflections | 34540 | |
| <I/σ(I)> | 8.9 | 1.4 |
| Completeness [%] | 96.5 | 81.7 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6 |
