4B3Z
Structure of the human collapsin response mediator protein-1, a lung cancer suppressor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-03 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.815, 147.846, 86.023 |
Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
Resolution | 39.184 - 3.050 |
R-factor | 0.1754 |
Rwork | 0.174 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1KCX AND 2VM8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.132 |
Data reduction software | HKL-2000 |
Phasing software | CCP4 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.080 | 0.440 |
Number of reflections | 42140 | |
<I/σ(I)> | 18.38 | 3.3 |
Completeness [%] | 99.6 | 91.1 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PROTEIN SOLUTION AT 10 MG/ML IN 20 MM TRIS-HCL WITH 150 MM NACL, PH 7.5 AND THE WELL SOLUTION (50 MM AMMONIUM SULFATE, 50 MM SODIUM CITRATE, 12-15% PEG 8000) IN A 1:1 RATIO |