4B3N
Crystal structure of rhesus TRIM5alpha PRY/SPRY domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-30 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.837, 98.733, 110.332 |
| Unit cell angles | 90.00, 122.99, 90.00 |
Refinement procedure
| Resolution | 49.420 - 3.300 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1ANF 2VOL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.416 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.170 | 0.890 |
| Number of reflections | 22400 | |
| <I/σ(I)> | 6.69 | 1.39 |
| Completeness [%] | 96.5 | 97.1 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 6% (W/V) GLUCOSE, 6% (W/V) TREHALOSE, 100 MM MES PH 6.2 AND 25% PEG 3350 |
