4B3A
Tetracycline repressor class D mutant H100A in complex with tetracycline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-17 |
Detector | RIGAKU CCD |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 67.537, 67.537, 179.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.800 - 1.700 |
R-factor | 0.2054 |
Rwork | 0.203 |
R-free | 0.24997 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vke |
RMSD bond length | 0.012 |
RMSD bond angle | 1.456 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.790 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.460 |
Number of reflections | 19362 | |
<I/σ(I)> | 20.4 | 0.9 |
Completeness [%] | 82.6 | 17.4 |
Redundancy | 5.86 | 1.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.5 M AMMONIUM SULPHATE, 1 M LITHIUM SULPHATE, 0.1 M MES PH 6.5 |