4B2P

RadA C-terminal ATPase domain from Pyrococcus furiosus bound to GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-05
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	40.320, 62.020, 87.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.590 - 1.600
R-factor	0.18068
Rwork	0.179
R-free	0.21976
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1PZN CHAIN A
RMSD bond length	0.026
RMSD bond angle	2.153
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.590	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.100	0.820
Number of reflections	29390
<I/σ(I)>	17.79	3.06
Completeness [%]	99.7	98.3
Redundancy	7.1	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.2		pH 6.2