4B23
Unprecedented sculpting of DNA at abasic sites by DNA glycosylase homolog Mag2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | RAXIS IV |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.480, 84.520, 125.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.2481 |
| Rwork | 0.248 |
| R-free | 0.28980 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.185 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 22272 | |
| <I/σ(I)> | 8.4 | 2.7 |
| Completeness [%] | 94.4 | 93.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.40 M (NH4) H2PO4 (NOT PH ADJUSTED), 6% 1,6-HEXANEDIOL |
