4B17

Crystal structure of C2498 2'-O-ribose methyltransferase RlmM from Escherichia coli in complex with S-adenosylmethionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-18
Detector	MARRESEARCH MAR165
Spacegroup name	P 31 2 1
Unit cell lengths	92.260, 92.260, 83.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.874 - 2.600
R-factor	0.1627
Rwork	0.161
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4atn
RMSD bond length	0.008
RMSD bond angle	1.137
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.870	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.100	0.640
Number of reflections	12983
<I/σ(I)>	16.37	3.27
Completeness [%]	99.8	99.9
Redundancy	7.4	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.3 M AMMONIUM TARTRATE DIBASIC PH 7.0, 20% PEG 3350, 3.0% DEXTRAN SULFATE SODIUM SALT