4B0Z

Crystal structure of S. pombe Rpn12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-17
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	41.780, 91.380, 143.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.111 - 1.585
R-factor	0.1898
Rwork	0.188
R-free	0.22480
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.525
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.340	1.670
High resolution limit [Å]	1.590	1.590
Rmerge	0.050	0.500
Number of reflections	74706
<I/σ(I)>	17.1	2.5
Completeness [%]	99.7	99.7
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.2 M NA NO3, 0.1 M BIS-TRIS PROPANE PH 7.5, 22.5% PEG3350