4AZZ

Carbohydrate binding module CBM66 from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-16
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	35.837, 60.950, 71.121
Unit cell angles	90.00, 100.89, 90.00

Refinement procedure

Resolution	69.840 - 1.700
R-factor	0.15986
Rwork	0.157
R-free	0.20628
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.014
RMSD bond angle	1.638
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.520
Number of reflections	32834
<I/σ(I)>	15	2.4
Completeness [%]	98.4	97.4
Redundancy	3.6	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN AT 20 MG/ML FROM 1 M LICL, 20% POLYETHYLENE 6000 AND 0.1 M CITRIC ACID, PH 4
1				PROTEIN AT 20 MG/ML FROM 1 M LICL, 20% POLYETHYLENE 6000 AND 0.1 M CITRIC ACID, PH 4