4AZH
Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.510, 115.970, 133.090 |
Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
Resolution | 43.710 - 2.220 |
R-factor | 0.19994 |
Rwork | 0.197 |
R-free | 0.25939 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yl5 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.461 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.030 | 2.340 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.150 | 0.600 |
Number of reflections | 99678 | |
<I/σ(I)> | 8.1 | 3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |