4AZ6
Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 78.300, 109.600, 112.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 78.560 - 1.360 |
R-factor | 0.1164 |
Rwork | 0.115 |
R-free | 0.13999 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yl6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.360 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.810 | 1.430 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.070 | 0.380 |
Number of reflections | 93267 | |
<I/σ(I)> | 19.1 | 4 |
Completeness [%] | 90.1 | 57.7 |
Redundancy | 9.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |