4AZ5
Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.540, 110.050, 112.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.570 - 1.730 |
| R-factor | 0.15796 |
| Rwork | 0.156 |
| R-free | 0.18629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yl6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.351 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.990 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 49487 | |
| <I/σ(I)> | 14.9 | 3.3 |
| Completeness [%] | 97.3 | 95 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
