Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 101.700, 101.700, 294.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.990 - 3.300 |
| R-factor | 0.2522 |
| Rwork | 0.251 |
| R-free | 0.28610 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD (SHARP) |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.130 | 0.360 |
| Number of reflections | 14186 | |
| <I/σ(I)> | 11 | 2.2 |
| Completeness [%] | 98.4 | 90.6 |
| Redundancy | 9.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 0.1 M SODIUM CHLORIDE, 5%(V/V)JEFFAMINE M-600, 0.1 M GLYCINE PH 9.5, 34%(V/V) PEG 400 CRYSTAL SOAKED BRIEFLY IN 10MM LUTETIUM CHLORIDE PRIOR TO DATA COLLECTION |
