4AYN
Structure of the C-terminal barrel of Neisseria meningitidis FHbp Variant 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.791, 75.951, 40.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.060 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w81 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.180 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.040 | 0.190 |
| Number of reflections | 14737 | |
| <I/σ(I)> | 26 | 6.8 |
| Completeness [%] | 98.5 | 91.3 |
| Redundancy | 5.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 30% PEG2KMME, 0.1 M SODIUM ACETATE PH 4.6, 0.2 M AMMONIUM SULPHATE |
