4AY6
Human O-GlcNAc transferase (OGT) in complex with UDP-5SGlcNAc and substrate peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 275.439, 275.439, 142.632 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.300 |
| R-factor | 0.22686 |
| Rwork | 0.227 |
| R-free | 0.27216 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.308 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.300 |
| Rmerge | 0.130 |
| Number of reflections | 91074 |
| <I/σ(I)> | 7.7 |
| Completeness [%] | 98.2 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.3 | 1.45 M K2HPO4, 10 MM EDTA, 1 % XYLITOL, pH 9.3 |
