4AY5
Human O-GlcNAc transferase (OGT) in complex with UDP and glycopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 274.170, 274.170, 142.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 237.440 - 3.150 |
| R-factor | 0.17398 |
| Rwork | 0.174 |
| R-free | 0.20391 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.036 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.150 |
| Rmerge | 0.110 |
| Number of reflections | 105108 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 99.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.3 | 1.45 M K2HPO4, 10 MM EDTA, 1% XYLITOL, pH 9.3 |
