4AXI
Structure of the Clostridium difficile EutS protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 123.200, 123.200, 38.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.930 - 1.510 |
| R-factor | 0.1417 |
| Rwork | 0.140 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cgi |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.679 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.600 | 1.590 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 34286 | |
| <I/σ(I)> | 12.4 | 2.5 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | PROTEIN IN 200 MM NACL, 50 MM TRIS HCL PH 8.0 HANGING DROP VAPOUR DIFFUSION WITH 100/100 NL PROTEIN: PRECIPITANT OVER 100 UL WELL SOLUTION OF 24% (W/V) PEG 1500, 20% (W/V) GLYCEROL |
