4AXG
Structure of eIF4E-Cup complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 142.580, 142.580, 108.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.314 - 2.800 |
| R-factor | 0.2291 |
| Rwork | 0.228 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u7x |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.653 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (MR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.310 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 1.140 |
| Number of reflections | 16494 | |
| <I/σ(I)> | 14.23 | 2.79 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.4 | 13.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350, 200 MM CALCIUM ACETATE |
