4AX2
New Type VI-secreted toxins and self-resistance proteins in Serratia marcescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-24 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.970, 77.970, 50.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.520 - 1.880 |
| R-factor | 0.18187 |
| Rwork | 0.180 |
| R-free | 0.21898 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.249 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.500 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 14813 | |
| <I/σ(I)> | 26.6 | 5.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.55M LI2SO4, 5% PEG 8000, pH 7.5 |
