4AX0
Q157A mutant. Crystal Structure of the Mobile Metallo-beta-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the role of Gln157
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 78.035, 78.035, 239.482 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.740 |
| R-factor | 0.12302 |
| Rwork | 0.121 |
| R-free | 0.15859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.578 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 43793 | |
| <I/σ(I)> | 0 | 2 |
| Completeness [%] | 96.2 | 76.4 |
| Redundancy | 4.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
