4AWX

Moonlighting functions of FeoC in the regulation of ferrous iron transport in Feo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 43
Unit cell lengths	59.690, 59.690, 141.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.520 - 2.300
R-factor	0.213
Rwork	0.213
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wia
RMSD bond length	0.009
RMSD bond angle	1.700
Data scaling software	SCALEPACK
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.400
Number of reflections	21747
<I/σ(I)>	28.02	3.05
Completeness [%]	98.9	92.1
Redundancy	4.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1