4AWJ

pVHL:EloB:EloC complex, in complex with capped Hydroxyproline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-28
Detector	DECTRIS PILATUS
Spacegroup name	P 41 2 2
Unit cell lengths	93.400, 93.400, 362.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.536 - 2.500
R-factor	0.2224
Rwork	0.220
R-free	0.26360
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.002
RMSD bond angle	0.634
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.110	0.760
Number of reflections	56728
<I/σ(I)>	17.34	3.25
Completeness [%]	99.9	100
Redundancy	12.6	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1 M NA CACODYLATE PH 6.0, 0.2 M MG ACETATE, 15% PEG3350, 5 MM DTT