4AW2
Crystal structure of CDC42 binding protein kinase alpha (MRCK alpha)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.744, 49.187, 97.762 |
| Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
| Resolution | 89.720 - 1.700 |
| R-factor | 0.17022 |
| Rwork | 0.169 |
| R-free | 0.19326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vd5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.910 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.900 |
| Number of reflections | 49832 | |
| <I/σ(I)> | 15.4 | 2.3 |
| Completeness [%] | 97.1 | 96.7 |
| Redundancy | 7.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2M KSCN, 0.1M BISTRISPROPANE PH 8.5, 20% PEG3350, 10% ETHYLENE GLYCOL |
