4AVF
Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-12-14 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 116.590, 116.590, 259.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.330 - 2.230 |
R-factor | 0.18045 |
Rwork | 0.179 |
R-free | 0.20003 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vrd |
RMSD bond length | 0.009 |
RMSD bond angle | 1.242 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.000 | 2.290 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.080 | 0.750 |
Number of reflections | 87734 | |
<I/σ(I)> | 18.5 | 3.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.1 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 11% PEG 4000, 0.1M MES PH 6.5 |